Cold-adapted variants of influenza A virus: evaluation in adult seronegative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60 strain.
Identifieur interne : 002881 ( Main/Exploration ); précédent : 002880; suivant : 002882Cold-adapted variants of influenza A virus: evaluation in adult seronegative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60 strain.
Auteurs : B R Murphy ; H P Holley ; E J Berquist ; M M Levine ; S B Spring ; H F Maassab ; A P Kendal ; R M ChanockSource :
- Infection and immunity [ 0019-9567 ] ; 1979.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Anticorps antiviraux (biosynthèse), Basse température, Grippe humaine (immunologie), Gènes viraux, Humains, Hémagglutinines virales, Recombinaison génétique, Vaccins antigrippaux (immunologie), Vaccins atténués (immunologie), Virus de la grippe A (génétique), Virus de la grippe A (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux.
- génétique : Virus de la grippe A.
- immunologie : Grippe humaine, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A.
- Adaptation physiologique, Basse température, Gènes viraux, Humains, Hémagglutinines virales, Recombinaison génétique.
English descriptors
- KwdEn :
- Adaptation, Physiological, Antibodies, Viral (biosynthesis), Cold Temperature, Genes, Viral, Hemagglutinins, Viral, Humans, Influenza A virus (genetics), Influenza A virus (immunology), Influenza Vaccines (immunology), Influenza, Human (immunology), Recombination, Genetic, Vaccines, Attenuated (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- genetics : Influenza A virus.
- immunology : Influenza A virus, Influenza Vaccines, Influenza, Human, Vaccines, Attenuated.
- Adaptation, Physiological, Cold Temperature, Genes, Viral, Hemagglutinins, Viral, Humans, Recombination, Genetic.
Abstract
Influenza A/Scotland/74 (H3N2) and A/Victoria/75 (H3N2) cold-adapted (ca) recombinant viruses, prepared by mating the A/Ann Arbor/6/60 (H2N2) ca donor virus and influenza A wild-type virus, were evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, =1:8) for level of attenuation, antigenicity, and genetic stability of the temperature-sensitive and ca phenotypes. At 10(7.0) to 10(7.5) 50% tissue culture infective doses the A/Scotland/74 and A/Victoria/75 ca recombinant viruses were clearly attenuated and antigenic. However, one of eight vaccinees infected with 10(7.5) 50% tissue culture infective doses of the A/Scotland/74 ca recombinant had a febrile reaction (39 degrees C). At a 10-fold higher dose (10(8.5) 50% tissue culture infective doses), 4 of 12 A/Scotland/74 vaccinees had a febrile and/or systemic reaction. Febrile reactions were not observed in volunteers who received the A/Victoria/75 ca recombinant virus, whereas 3 of the 12 vaccinees had mild upper respiratory tract symptoms, in one instance associated with mild systemic manifestations. Significantly, the serum hemagglutination- and neuraminidase-inhibiting antibody responses were comparable to those induced by wild-type virus. Both ca recombinant viruses were shed in low titer for a short period of time. Each isolate retained the temperature-sensitive phenotype. However, there was evidence of genetic instability of the ca marker in that 7 of 24 isolates exhibited some loss of the ca property, and one isolate completely lost the capacity to produce plaques at 25 degrees C. The retention of a low level of residual reactogenicity in the A/Scotland/74 ca recombinant suggests that acquisition of the ca and temperature-sensitive phenotypes by a ca recombinant virus may not always bring about a satisfactory level of attenuation for individuals lacking hemagglutinin immunity.
PubMed: 422240
Affiliations:
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Le document en format XML
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<term>Genes, Viral</term>
<term>Hemagglutinins, Viral</term>
<term>Humans</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (immunology)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Recombination, Genetic</term>
<term>Vaccines, Attenuated (immunology)</term>
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<term>Anticorps antiviraux (biosynthèse)</term>
<term>Basse température</term>
<term>Grippe humaine (immunologie)</term>
<term>Gènes viraux</term>
<term>Humains</term>
<term>Hémagglutinines virales</term>
<term>Recombinaison génétique</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins atténués (immunologie)</term>
<term>Virus de la grippe A (génétique)</term>
<term>Virus de la grippe A (immunologie)</term>
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<term>Vaccins antigrippaux</term>
<term>Vaccins atténués</term>
<term>Virus de la grippe A</term>
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<term>Influenza Vaccines</term>
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<term>Vaccines, Attenuated</term>
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<term>Recombination, Genetic</term>
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<term>Hémagglutinines virales</term>
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<front><div type="abstract" xml:lang="en">Influenza A/Scotland/74 (H3N2) and A/Victoria/75 (H3N2) cold-adapted (ca) recombinant viruses, prepared by mating the A/Ann Arbor/6/60 (H2N2) ca donor virus and influenza A wild-type virus, were evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, =1:8) for level of attenuation, antigenicity, and genetic stability of the temperature-sensitive and ca phenotypes. At 10(7.0) to 10(7.5) 50% tissue culture infective doses the A/Scotland/74 and A/Victoria/75 ca recombinant viruses were clearly attenuated and antigenic. However, one of eight vaccinees infected with 10(7.5) 50% tissue culture infective doses of the A/Scotland/74 ca recombinant had a febrile reaction (39 degrees C). At a 10-fold higher dose (10(8.5) 50% tissue culture infective doses), 4 of 12 A/Scotland/74 vaccinees had a febrile and/or systemic reaction. Febrile reactions were not observed in volunteers who received the A/Victoria/75 ca recombinant virus, whereas 3 of the 12 vaccinees had mild upper respiratory tract symptoms, in one instance associated with mild systemic manifestations. Significantly, the serum hemagglutination- and neuraminidase-inhibiting antibody responses were comparable to those induced by wild-type virus. Both ca recombinant viruses were shed in low titer for a short period of time. Each isolate retained the temperature-sensitive phenotype. However, there was evidence of genetic instability of the ca marker in that 7 of 24 isolates exhibited some loss of the ca property, and one isolate completely lost the capacity to produce plaques at 25 degrees C. The retention of a low level of residual reactogenicity in the A/Scotland/74 ca recombinant suggests that acquisition of the ca and temperature-sensitive phenotypes by a ca recombinant virus may not always bring about a satisfactory level of attenuation for individuals lacking hemagglutinin immunity.</div>
</front>
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<tree><noCountry><name sortKey="Berquist, E J" sort="Berquist, E J" uniqKey="Berquist E" first="E J" last="Berquist">E J Berquist</name>
<name sortKey="Chanock, R M" sort="Chanock, R M" uniqKey="Chanock R" first="R M" last="Chanock">R M Chanock</name>
<name sortKey="Holley, H P" sort="Holley, H P" uniqKey="Holley H" first="H P" last="Holley">H P Holley</name>
<name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name>
<name sortKey="Levine, M M" sort="Levine, M M" uniqKey="Levine M" first="M M" last="Levine">M M Levine</name>
<name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name>
<name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name>
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